Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and. Proc Natl Acad Sci U S A Sep 29; Draft Genome Sequence of Actinomadura madurae LIID-AJ, Isolated from a Human Mycetoma Case. Vera-Cabrera L.

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Nucleotide sequence accession number. Deep sequencing has previously been used to characterize cultured bacterial isolates 27 and to explore the composition of microbial populations in metagenomic research 28 Culture of discharged material reportedly returned with overgrowth of skin flora and provided no mafurae of infection with Actinomyces species. Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis.

The tallies and percent abundance of reads corresponding to each organism are indicated. Departments of Laboratory Medicine a.

Actinomadura madurae (ID ) – Genome – NCBI

It contains aerobic, Gram-positive, non-acid-fast, non-motile, chemo-organotrophic actinomycetes that produce well-developed, non-fragmenting vegetative mycelia and aerial hyphae that differentiate into surface-ornamented spore chains.

Cooksona, b and Noah G. Int J Syst Evol Microbiol.

A two-step madurrae for the treatment of actinomycotic mycetomas. Please review our privacy policy. The case exemplifies several capabilities of deep sequencing as a clinical diagnostic tool, specifically, deconvoluting the identity of individual organisms within polymicrobial samples, classifying organisms directly from patient specimens without the need for culture, and characterizing nonviable or unculturable organisms in this case, organisms killed by FFPE processing prior to pathology examination.


Associated Actinomaduura Supplementary Materials. In this case report, we have utilized the approach to investigate the causative agent of biopsy-proven actinomycotic mycetoma.

Nonmycetomic Actinomadura madurae infection in a patient with AIDS.

This Actinobacteria -related article is a stub. The patient was referred to the Infectious Disease clinic at Harborview Medical Center for further evaluation. Several treatment regimens for actinomycotic mycetoma have been published 18 — 20and in general, the condition shows response to a wide range of antibiotics, including aminoglycosides, rifampin, amoxicillin-clavulanic acid, doxycycline, and sulfamethoxazole-trimethoprim, although combination antibiotic therapy is recommended Table 1 details the classification of deep-sequencing reads from the specimen submitted for analysis.

Polymicrobial specimens may be difficult or even impossible to fully characterize by techniques in common clinical use: The diagnosis of A. This pathogen is one of the classical agents of actinomycotic mycetoma and is consistent with the organism visualized histologically. Hoogestraata Lisa A. Fournier PE, Raoult D.

The Ribosomal Database Project: Many sites of the human body are colonized by actibomadura communities of microbes in both health and various disease states 6. From Wikipedia, the free encyclopedia.


Comparison of DNA extraction methods for microbial community profiling with an application to pediatric bronchoalveolar lavage samples. Actinomycetoma in the United States. The patient agreed to this procedure, and was discharged on a course of oral sulfamethoxazole-trimethoprim.

Neoscytalidium dimidiatum and Aspergillus actinmoadura. Footnotes Published ahead of print 9 October Next-generation sequencing in clinical microbiology. Both of these diagnostic limitations complicated analysis of the patient specimen in this report, a biopsy specimen from a diabetic foot ulcer. By using this site, you agree to the Terms of Use and Privacy Policy. Bacterial community shift in treated periodontitis patients revealed by ion torrent 16S rRNA gene amplicon sequencing.

Info – Actinomadura madurae DSM

The consensus sequence determined in this work has marurae submitted to GenBank under accession no. A systematic approach for discovering novel, clinically relevant bacteria. Table 1 Deep-sequencing results.

Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions. Given the extent of infection, neither additional antibiotic therapy nor debridement with subsequent reconstruction was felt to be a viable option, and a recommendation was made for below-the-knee amputation.