ISO 20776 PDF

ISO 20776 PDF

ISO. First edition. Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation. ISO () Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of. Buy ISO , Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices, Part 1, at CLSI.

Author: Shataxe Aralar
Country: Cyprus
Language: English (Spanish)
Genre: Photos
Published (Last): 8 August 2005
Pages: 212
PDF File Size: 13.79 Mb
ePub File Size: 6.98 Mb
ISBN: 791-8-29578-942-2
Downloads: 73513
Price: Free* [*Free Regsitration Required]
Uploader: Mikus

General Committee to Susceptibility testing of moulds International Organization for Standards, Geneva, Switzerland.

EUCAST: External documents

Microtiter plates were prepared according to the ISO standard and by serial dilution procedures recommended for preparation of trays of both hydrophilic and hydrophobic drugs 9 Single-step extraction of fluconazole from plasma by ultra-filtration for the measurement of its free concentration by high performance liquid chromatography.

Fluconazole trays can be accurately prepared by following ISO and serial dilution schemes. Example of a quick liquid chromatographic assay of itraconazole in serum. First indications of improved capability is capacity of public health microbiology laboratories in the European Union http: FLC eluted at 4.

Relevant external documents

Selective high-performance liquid chromatographic assay 02776 itraconazole and hydroxyitraconazole in plasma from human immunodeficiency virus-infected patients. National Center for Biotechnology InformationU. While this is the gold standard for preparation of dilution series, the question 207776 risen if modern pipettes are sufficiently precise to allow dilution series to be prepared as serial dilutions without a loss of accuracy. In summary, our data suggest that microdilution trays for hydrophilic drugs such as FLC can be accurately prepared following the ISO recommendations or the serial 2-fold dilution iao.

Projects and data submission Received Jan 20; Accepted Feb Thus, ITC data obtained from trays prepared by the Inn Austria participant were excluded from the analysis since tray wrappings showed evidence of defrosting and spillage; also, concentrations of dilutions prepared in Denmark were unacceptably different from the target concentrations. Considering only data for dilution series prepared in Spain, no systematic difference was detected between ISO dilution and serial dilution.

  KALI THE BLACK GODDESS OF DAKSHINESWAR BY ELIZABETH HARDING PDF

Fifty microliters of the resultant mix was injected directly into the HPLC system. 20767 method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases.

Simultaneous quantification of voriconazole and posaconazole in human plasma by high-performance liquid chromatography with ultra-violet detection.

Regarding serial 2-fold dilution procedures, better results were detected when FLC plates were prepared according to the hydrophilic rather than the hydrophobic way. Precision of itraconazole dilution series by procedure and laboratory a.

Prevalidation statistical design to assess analytical methods. HPLC is a sensitive and reproducible method for determining levels of azole components in a great variety of matrices, as has been confirmed in previous reports 12510 – 121519 – 21 Precision of fluconazole dilution series by dilution procedure and laboratory a. Rapid AST in bloodcultures ISO Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices – Part 1: Previous versions of documents Veterinary Susceptibility Testing Susceptibility testing of yeasts All is analyses were done with the statistical package PAWS statistics This article has been cited by other articles in PMC.

However, itraconazole trays showed a significant lack of reproducibility that was independent of which method was followed. 2076

Reference method for broth dilution antifungal susceptibility testing of yeast, 3rd ed. Since the hydrophilic growth medium RPMI-G at pH 7 has been selected for testing in vitro antifungal susceptibility, it was important to 207766 the lack of proper solubility of this compound. ISO Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices — Part 2: Trays were done in duplicate on separate days at each center and then shipped frozen by special delivery to Spain for further chromatographic concentration determinations.

  48070 SCHNEIDER PDF

Bioanalytical method validation-a revisit with a decade of progress. Quality control procedures of AST should be implemented in order to minimize the inaccuracy of dilution methods and lack of reproducibility when hydrophobic 207766 such as ITC are tested. Please review our privacy policy. Meetings, Minutes and Reports Thus, serial dilution, which is attractive due to its simplicity, may be appropriate.

You are lso to check costs as they may differ substantially between suppliers. Development and validation of a high-performance iiso chromatographic assay for the determination of fluconazole in human whole blood using solid phase extraction.

Splitting wild type distributions. Where clinical data is lacking! Susceptibility testing of pathogenic fungi with itraconazole: Contact Sitemap Privacy Statement Disclaimer. Notably, although relative errors were greater for the lowest concentrations, we did not observe an increasing inaccuracy when performing serial dilutions compared to dilutions performed following the ISO procedure as would have been expected if the precision of 27076 pipettes had been insufficient.

Reference method for broth dilution antifungal susceptibility testing of filamentous fungi, 2nd ed. ITC precipitation after thaw-freeze cycles during transport is the most probable explanation. ISO documents of relevance to antimicrobial susceptibility testing These documents are available for purchase from ISO or national standards organisations.